For Research Use Only. Not for Clinical Use.

Many biotherapeutics have the shortcoming of a short half-life. Various half-life extension strategies are available to extend the half-life of the therapeutic molecules. The strategies aim at reducing renal clearance, mainly by increasing the hydrodynamic volume, which can be achieved through posttranslational modifications or genetically fusing half-life extension moieties to the protein. Creative Biolabs has years of experience in half-life extension. We can provide a variety of services of half-life extension.

Introduction of Gene Fusion Therapeutic Molecules to Albumin

Therapeutic compounds have been generated by gene fusion where the gene for a protein is linked to that of albumin and expressed from a suitable expression host to produce a single polypeptide. In the past decade, numerous proteins have been genetically fused to albumin. Compared with the unfused counterparts, the fused biologically active molecules have a longer half-life. The fused biologically active molecules included peptides, cytokines, enzymes, hormones, coagulation factors, growth factors, various antibody fragments, and redox modulators.

Fig.1 Schematic model of gene fusion. (Latysheva, 2016)

While most albumin fusions comprise a single therapeutic protein fused to albumin, it is also possible to construct bispecific and bivalent albumin fusions, where therapeutic molecules can be fused to both the N- and the C-termini of albumin. Other methods include the direct fusion to serum albumins, such as utilized for glucagonlike peptide-1 and glycoengineering. In addition, flexible polypeptide chains represent an alternative to conjugation technology providing biocompatible and biodegradable structures, which can be added to the therapeutic molecules by genetic fusion either to the N-terminus or C-terminus or to both ends. Various polypeptide sequences have been developed in recent years. These fusions have been made from a variety of expression platforms. The half-life of the original therapeutic protein will have an important effect on the half-life of albumin fusion ones.

Advantages

• Suitable for various therapeutic molecules
• Attached through genetic fusion avoiding chemical coupling
• Remove the steps of conjugation and post-conjugation clean
• Lower manufacturing cost

Our Services of Half-Life Extension Based on Genetic Fusion

Based on the above advantages, gene fusion is emerging as a ponderable in half-life extension. Creative Biolabs has built an advanced gene fusion platform, and our scientists have professional knowledge of various forms of gene fusion. We can offer a variety of gene fusion services, which including but not limited to genetic fusion peptides and protein albumin, genetic fusion cytokines, growth factors, albumin and enzyme hormones, gene fusion redox regulators, and albumin antibody fragments.

Creative Biolabs has the most advanced technology platform and the most professional experts in the field of half-life extension. Based on rich experience and profound background knowledge, we can provide the best therapeutic molecules to accelerate your project. Please contact us to learn more about our services.

Reference

1. Latysheva, N. S.; et al. Molecular principles of gene fusion mediated rewiring of protein interaction networks in cancer. Molecular cell. 2016, 63(4): 579-592.